Publications

Intra-species diversity of Clostridium perfringens: A diverse genetic repertoire reveals its pathogenic potential. Camargo A, Guerrero-Araya E, Castañeda S, Vega L, Cardenas-Alvarez MX, Rodríguez C, Paredes-Sabja D, Ramírez JD, Muñoz M.Front Microbiol. 2022 Jul 22;13:952081. doi: 10.3389/fmicb.2022.952081. eCollection 2022.PMID: 35935202 Free PMC article.

Author Correction: Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Castro-Córdova P, Mora-Uribe P, Reyes-Ramírez R, Cofré-Araneda G, Orozco-Aguilar J, Brito-Silva C, Mendoza-León MJ, Kuehne SA, Minton NP, Pizarro-Guajardo M, Paredes-Sabja D.Nat Commun. 2022 Apr 29;13(1):2465. doi: 10.1038/s41467-022-30283-x.PMID: 35487940 Free PMC article. No abstract available.

Assembly of the exosporium layer in Clostridioides difficile spores. Paredes-Sabja D, Cid-Rojas F, Pizarro-Guajardo M.Curr Opin Microbiol. 2022 Jun;67:102137. doi: 10.1016/j.mib.2022.01.008. Epub 2022 Feb 16.PMID: 35182899 Review.

Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice. Castro-Córdova P, Mendoza-León MJ, Paredes-Sabja D.PLoS One. 2021 Dec 22;16(12):e0261081. doi: 10.1371/journal.pone.0261081. eCollection 2021.PMID: 34936648 Free PMC article.

FastMLST: A Multi-core Tool for Multilocus Sequence Typing of Draft Genome Assemblies. Guerrero-Araya E, Muñoz M, Rodríguez C, Paredes-Sabja D.Bioinform Biol Insights. 2021 Nov 27;15:11779322211059238. doi: 10.1177/11779322211059238. eCollection 2021.PMID: 34866905 Free PMC article.

Landscapes and bacterial signatures of mucosa-associated intestinal microbiota in Chilean and Spanish patients with inflammatory bowel disease. Chamorro N, Montero DA, Gallardo P, Farfán M, Contreras M, De la Fuente M, Dubois K, Hermoso MA, Quera R, Pizarro-Guajardo M, Paredes-Sabja D, Ginard D, Rosselló-Móra R, Vidal R.Microb Cell. 2021 Jun 18;8(9):223-238. doi: 10.15698/mic2021.09.760. eCollection 2021 Sep 6.PMID: 34527721 Free PMC article.

Updating changes in human gut microbial communities associated with Clostridioides difficile infection. Herrera G, Paredes-Sabja D, Patarroyo MA, Ramírez JD, Muñoz M.Gut Microbes. 2021 Jan-Dec;13(1):1966277. doi: 10.1080/19490976.2021.1966277.PMID: 34486488 Free PMC article. Review.

Evolution and Epidemic Spread of SARS-CoV-2 in Colombia: A Year into the Pandemic. Castañeda S, Patiño LH, Muñoz M, Ballesteros N, Guerrero-Araya E, Paredes-Sabja D, Flórez C, Gomez S, Ramírez-Santana C, Salguero G, Gallo JE, Paniz-Mondolfi AE, Ramírez JD.Vaccines (Basel). 2021 Jul 30;9(8):837. doi: 10.3390/vaccines9080837.PMID: 34451962 Free PMC article.

Major genetic discontinuity and novel toxigenic species in Clostridioides difficile taxonomy. Knight DR, Imwattana K, Kullin B, Guerrero-Araya E, Paredes-Sabja D, Didelot X, Dingle KE, Eyre DW, Rodríguez C, Riley TV.Elife. 2021 Jun 11;10:e64325. doi: 10.7554/eLife.64325.PMID: 34114561 Free PMC article.

Clostridioides difficile spores stimulate inflammatory cytokine responses and induce cytotoxicity in macrophages. Chiu PJ, Rathod J, Hong YP, Tsai PJ, Hung YP, Ko WC, Chen JW, Paredes-Sabja D, Huang IH.Anaerobe. 2021 Aug;70:102381. doi: 10.1016/j.anaerobe.2021.102381. Epub 2021 May 31.PMID: 34082120

Visualization of fidaxomicin association with the exosporium layer of Clostridioides difficile spores. Bassères E, Endres BT, Montes-Bravo N, Pérez-Soto N, Rashid T, Lancaster C, Begum K, Alam MJ, Paredes-Sabja D, Garey KW.Anaerobe. 2021 Jun;69:102352. doi: 10.1016/j.anaerobe.2021.102352. Epub 2021 Feb 25.PMID: 33640461 Free article.

Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Castro-Córdova P, Mora-Uribe P, Reyes-Ramírez R, Cofré-Araneda G, Orozco-Aguilar J, Brito-Silva C, Mendoza-León MJ, Kuehne SA, Minton NP, Pizarro-Guajardo M, Paredes-Sabja D.Nat Commun. 2021 Feb 18;12(1):1140. doi: 10.1038/s41467-021-21355-5.PMID: 33602902 Free PMC article.

The Clostridioides difficile Cysteine-Rich Exosporium Morphogenetic Protein, CdeC, Exhibits Self-Assembly Properties That Lead to Organized Inclusion Bodies in Escherichia coli. Romero-Rodríguez A, Troncoso-Cotal S, Guerrero-Araya E, Paredes-Sabja D.mSphere. 2020 Nov 18;5(6):e01065-20. doi: 10.1128/mSphere.01065-20.PMID: 33208520 Free PMC article.

Comprehensive genome analyses of Sellimonas intestinalis, a potential biomarker of homeostasis gut recovery. Muñoz M, Guerrero-Araya E, Cortés-Tapia C, Plaza-Garrido A, Lawley TD, Paredes-Sabja D.Microb Genom. 2020 Dec;6(12):mgen000476. doi: 10.1099/mgen.0.000476. Epub 2020 Nov 18.PMID: 33206037 Free PMC article.

Nasal Immunization with the C-Terminal Domain of Bcla3 Induced Specific IgG Production and Attenuated Disease Symptoms in Mice Infected with Clostridioides difficile Spores. Maia AR, Reyes-Ramírez R, Pizarro-Guajardo M, Saggese A, Ricca E, Baccigalupi L, Paredes-Sabja D.Int J Mol Sci. 2020 Sep 13;21(18):6696. doi: 10.3390/ijms21186696.PMID: 32933117 Free PMC article.

Characterization of Exosporium Layer Variability of Clostridioides difficile Spores in the Epidemically Relevant Strain R20291. Pizarro-Guajardo M, Calderón-Romero P, Romero-Rodríguez A, Paredes-Sabja D.Front Microbiol. 2020 Jul 2;11:1345. doi: 10.3389/fmicb.2020.01345. eCollection 2020.PMID: 32714296 Free PMC article.

Evaluation of functionality of type II toxin-antitoxin systems of Clostridioides difficile R20291. Álvarez R, Ortega-Fuentes C, Queraltó C, Inostroza O, Díaz-Yáñez F, González R, Calderón IL, Fuentes JA, Paredes-Sabja D, Gil F.Microbiol Res. 2020 Oct;239:126539. doi: 10.1016/j.micres.2020.126539. Epub 2020 Jun 28.PMID: 32622285 Free article.

Origin, genomic diversity and microevolution of the Clostridium difficile B1/NAP1/RT027/ST01 strain in Costa Rica, Chile, Honduras and Mexico. Guerrero-Araya E, Meneses C, Castro-Nallar E, Guzmán D AM, Álvarez-Lobos M, Quesada-Gómez C, Paredes-Sabja D, Rodríguez C.Microb Genom. 2020 May;6(5):e000355. doi: 10.1099/mgen.0.000355. Epub 2020 Mar 16.PMID: 32176604 Free PMC article.

Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2_ctd of Clostridioides difficile. Maia AR, Reyes-Ramírez R, Pizarro-Guajardo M, Saggese A, Castro-Córdova P, Isticato R, Ricca E, Paredes-Sabja D, Baccigalupi L.Int J Mol Sci. 2020 Feb 14;21(4):1277. doi: 10.3390/ijms21041277.PMID: 32074955 Free PMC article.

Effect of antibiotic to induce Clostridioides difficile-susceptibility and infectious strain in a mouse model of Clostridioides difficile infection and recurrence. Castro-Córdova P, Díaz-Yáñez F, Muñoz-Miralles J, Gil F, Paredes-Sabja D.Anaerobe. 2020 Apr;62:102149. doi: 10.1016/j.anaerobe.2020.102149. Epub 2020 Jan 12.PMID: 31940467

Sporulation and Germination in Clostridial Pathogens. Shen A, Edwards AN, Sarker MR, Paredes-Sabja D.Microbiol Spectr. 2019 Nov;7(6):10.1128/microbiolspec.GPP3-0017-2018. doi: 10.1128/microbiolspec.GPP3-0017-2018.PMID: 31858953 Free PMC article. Review.

cfr(B), cfr(C), and a New cfr-Like Gene, cfr(E), in Clostridium difficile Strains Recovered across Latin America. Stojković V, Ulate MF, Hidalgo-Villeda F, Aguilar E, Monge-Cascante C, Pizarro-Guajardo M, Tsai K, Tzoc E, Camorlinga M, Paredes-Sabja D, Quesada-Gómez C, Fujimori DG, Rodríguez C.Antimicrob Agents Chemother. 2019 Dec 20;64(1):e01074-19. doi: 10.1128/AAC.01074-19. Print 2019 Dec 20.PMID: 31685464 Free PMC article.

New insights for vaccine development against Clostridium difficile infections. Pizarro-Guajardo M, Chamorro-Veloso N, Vidal RM, Paredes-Sabja D.Anaerobe. 2019 Aug;58:73-79. doi: 10.1016/j.anaerobe.2019.04.009. Epub 2019 Apr 26.PMID: 31034928 Review.

Clostridium difficile toxins induce VEGF-A and vascular permeability to promote disease pathogenesis. Huang J, Kelly CP, Bakirtzi K, Villafuerte Gálvez JA, Lyras D, Mileto SJ, Larcombe S, Xu H, Yang X, Shields KS, Zhu W, Zhang Y, Goldsmith JD, Patel IJ, Hansen J, Huang M, Yla-Herttuala S, Moss AC, Paredes-Sabja D, Pothoulakis C, Shah YM, Wang J, Chen X.Nat Microbiol. 2019 Feb;4(2):269-279. doi: 10.1038/s41564-018-0300-x. Epub 2018 Dec 3.PMID: 30510170 Free PMC article.

Identification of Escherichia coli strains for the heterologous overexpression of soluble Clostridium difficile exosporium proteins. Brito-Silva C, Pizarro-Cerda J, Gil F, Paredes-Sabja D.J Microbiol Methods. 2018 Nov;154:46-51. doi: 10.1016/j.mimet.2018.10.002. Epub 2018 Oct 4.PMID: 30291882

Indomethacin increases severity of Clostridium difficile infection in mouse model. Muñoz-Miralles J, Trindade BC, Castro-Córdova P, Bergin IL, Kirk LA, Gil F, Aronoff DM, Paredes-Sabja D.Future Microbiol. 2018 Sep;13(11):1271-1281. doi: 10.2217/fmb-2017-0311. Epub 2018 Sep 21.PMID: 30238771 Free PMC article.

Clostridium difficile exosporium cysteine-rich proteins are essential for the morphogenesis of the exosporium layer, spore resistance, and affect C. difficile pathogenesis. Calderón-Romero P, Castro-Córdova P, Reyes-Ramírez R, Milano-Céspedes M, Guerrero-Araya E, Pizarro-Guajardo M, Olguín-Araneda V, Gil F, Paredes-Sabja D.PLoS Pathog. 2018 Aug 8;14(8):e1007199. doi: 10.1371/journal.ppat.1007199. eCollection 2018 Aug.PMID: 30089172 Free PMC article.

Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells. Álvarez R, Inostroza O, Garavaglia M, Minton NP, Paredes-Sabja D, Gil F.J Antimicrob Chemother. 2018 Sep 1;73(9):2396-2399. doi: 10.1093/jac/dky186.PMID: 29757406

Subtyping of Clostridium difficile PCR ribotypes 591, 106 and 002, the dominant strain types circulating in Medellin, Colombia. Salazar CL, Reyes C, Cienfuegos-Gallet AV, Best E, Atehortua S, Sierra P, Correa MM, Fawley WN, Paredes-Sabja D, Wilcox M, Gonzalez A.PLoS One. 2018 Apr 12;13(4):e0195694. doi: 10.1371/journal.pone.0195694. eCollection 2018.PMID: 29649308 Free PMC article.

Identification of Clostridium difficile Immunoreactive Spore Proteins of the Epidemic Strain R20291. Pizarro-Guajardo M, Ravanal MC, Paez MD, Callegari E, Paredes-Sabja D.Proteomics Clin Appl. 2018 Sep;12(5):e1700182. doi: 10.1002/prca.201700182. Epub 2018 Apr 18.PMID: 29573213 Free PMC article.

Lauric Acid Is an Inhibitor of Clostridium difficile Growth in Vitro and Reduces Inflammation in a Mouse Infection Model. Yang HT, Chen JW, Rathod J, Jiang YZ, Tsai PJ, Hung YP, Ko WC, Paredes-Sabja D, Huang IH.Front Microbiol. 2018 Jan 17;8:2635. doi: 10.3389/fmicb.2017.02635. eCollection 2017.PMID: 29387044 Free PMC article.

Inactivation model and risk-analysis design for apple juice processing by high-pressure CO₂. Deng K, Serment-Moreno V, Welti-Chanes J, Paredes-Sabja D, Fuentes C, Wu X, Torres JA.J Food Sci Technol. 2018 Jan;55(1):258-264. doi: 10.1007/s13197-017-2933-9. Epub 2017 Nov 16.PMID: 29358818 Free PMC article.

Effect of microalgae on intestinal inflammation triggered by soybean meal and bacterial infection in zebrafish. Bravo-Tello K, Ehrenfeld N, Solís CJ, Ulloa PE, Hedrera M, Pizarro-Guajardo M, Paredes-Sabja D, Feijóo CG.PLoS One. 2017 Nov 8;12(11):e0187696. doi: 10.1371/journal.pone.0187696. eCollection 2017.PMID: 29117213 Free PMC article.

Molecular, microbiological and clinical characterization of Clostridium difficile isolates from tertiary care hospitals in Colombia. Salazar CL, Reyes C, Atehortua S, Sierra P, Correa MM, Paredes-Sabja D, Best E, Fawley WN, Wilcox M, González Á.PLoS One. 2017 Sep 13;12(9):e0184689. doi: 10.1371/journal.pone.0184689. eCollection 2017.PMID: 28902923 Free PMC article.

Characterization of Chicken IgY Specific to Clostridium difficile R20291 Spores and the Effect of Oral Administration in Mouse Models of Initiation and Recurrent Disease. Pizarro-Guajardo M, Díaz-González F, Álvarez-Lobos M, Paredes-Sabja D.Front Cell Infect Microbiol. 2017 Aug 14;7:365. doi: 10.3389/fcimb.2017.00365. eCollection 2017.PMID: 28856119 Free PMC article.

Survival of Clostridium difficile spores at low water activity. Deng K, Talukdar PK, Sarker MR, Paredes-Sabja D, Torres JA.Food Microbiol. 2017 Aug;65:274-278. doi: 10.1016/j.fm.2017.03.013. Epub 2017 Mar 20.PMID: 28400013

Updates on Clostridium difficile spore biology. Gil F, Lagos-Moraga S, Calderón-Romero P, Pizarro-Guajardo M, Paredes-Sabja D.Anaerobe. 2017 Jun;45:3-9. doi: 10.1016/j.anaerobe.2017.02.018. Epub 2017 Feb 22.PMID: 28254263 Review.

Genome Sequence of Clostridium paraputrificum 373-A1 Isolated in Chile from a Patient Infected with Clostridium difficile. Guerrero-Araya E, Plaza-Garrido A, Díaz-Yañez F, Pizaro-Guajardo M, Valenzuela SL, Meneses C, Gil F, Castro-Nallar E, Paredes-Sabja D.Genome Announc. 2016 Nov 3;4(6):e01178-16. doi: 10.1128/genomeA.01178-16.PMID: 27811092 Free PMC article.

Lose to win: marT pseudogenization in Salmonella enterica serovar Typhi contributed to the surV-dependent survival to H₂O₂, and inside human macrophage-like cells. Ortega AP, Villagra NA, Urrutia IM, Valenzuela LM, Talamilla-Espinoza A, Hidalgo AA, Rodas PI, Gil F, Calderón IL, Paredes-Sabja D, Mora GC, Fuentes JA.Infect Genet Evol. 2016 Nov;45:111-121. doi: 10.1016/j.meegid.2016.08.029. Epub 2016 Aug 24.PMID: 27567490

Characterization of the Adherence of Clostridium difficile Spores: The Integrity of the Outermost Layer Affects Adherence Properties of Spores of the Epidemic Strain R20291 to Components of the Intestinal Mucosa. Mora-Uribe P, Miranda-Cárdenas C, Castro-Córdova P, Gil F, Calderón I, Fuentes JA, Rodas PI, Banawas S, Sarker MR, Paredes-Sabja D.Front Cell Infect Microbiol. 2016 Sep 22;6:99. doi: 10.3389/fcimb.2016.00099. eCollection 2016.PMID: 27713865 Free PMC article.

Characterization of germinants and their receptors for spores of non-food-borne Clostridium perfringens strain F4969. Banawas S, Paredes-Sabja D, Setlow P, Sarker MR.Microbiology (Reading). 2016 Nov;162(11):1972-1983. doi: 10.1099/mic.0.000378. Epub 2016 Sep 29.PMID: 27692042

A feed-forward loop between SroC and MgrR small RNAs modulates the expression of eptB and the susceptibility to polymyxin B in Salmonella Typhimurium. Acuña LG, Barros MJ, Peñaloza D, Rodas PI, Paredes-Sabja D, Fuentes JA, Gil F, Calderón IL.Microbiology (Reading). 2016 Nov;162(11):1996-2004. doi: 10.1099/mic.0.000365. Epub 2016 Aug 26.PMID: 27571709

Acyldepsipeptide antibiotics as a potential therapeutic agent against Clostridium difficile recurrent infections. Gil F, Paredes-Sabja D.Future Microbiol. 2016 Sep;11:1179-89. doi: 10.2217/fmb-2016-0064. Epub 2016 Aug 22.PMID: 27546386 Review.

Salmonella Typhimurium exhibits fluoroquinolone resistance mediated by the accumulation of the antioxidant molecule H2S in a CysK-dependent manner. Frávega J, Álvarez R, Díaz F, Inostroza O, Tejías C, Rodas PI, Paredes-Sabja D, Fuentes JA, Calderón IL, Gil F.J Antimicrob Chemother. 2016 Dec;71(12):3409-3415. doi: 10.1093/jac/dkw311. Epub 2016 Aug 15.PMID: 27530757

Ultrastructure Variability of the Exosporium Layer of Clostridium difficile Spores from Sporulating Cultures and Biofilms. Pizarro-Guajardo M, Calderón-Romero P, Paredes-Sabja D.Appl Environ Microbiol. 2016 Sep 16;82(19):5892-8. doi: 10.1128/AEM.01463-16. Print 2016 Oct 1.PMID: 27474709 Free PMC article.

The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion. Rodas PI, Álamos-Musre AS, Álvarez FP, Escobar A, Tapia CV, Osorio E, Otero C, Calderón IL, Fuentes JA, Gil F, Paredes-Sabja D, Christodoulides M.FEMS Microbiol Lett. 2016 Sep;363(17):fnw181. doi: 10.1093/femsle/fnw181. Epub 2016 Jul 26.PMID: 27465490 Free PMC article.

Clostridium perfringens Sporulation and Sporulation-Associated Toxin Production. Li J, Paredes-Sabja D, Sarker MR, McClane BA.Microbiol Spectr. 2016 Jun;4(3):10.1128/microbiolspec.TBS-0022-2015. doi: 10.1128/microbiolspec.TBS-0022-2015.PMID: 27337447 Free PMC article. Review.

Ultrastructural Variability of the Exosporium Layer of Clostridium difficile Spores. Pizarro-Guajardo M, Calderón-Romero P, Castro-Córdova P, Mora-Uribe P, Paredes-Sabja D.Appl Environ Microbiol. 2016 Feb 5;82(7):2202-2209. doi: 10.1128/AEM.03410-15.PMID: 26850296 Free PMC article.

Predominance of Clostridium difficile ribotypes 012, 027 and 046 in a university hospital in Chile, 2012. Plaza-Garrido Á, Barra-Carrasco J, Macias JH, Carman R, Fawley WN, Wilcox MH, Hernández-Rocha C, Guzmán-Durán AM, Alvarez-Lobos M, Paredes-Sabja D.Epidemiol Infect. 2016 Apr;144(5):976-9. doi: 10.1017/S0950268815002459. Epub 2015 Oct 22.PMID: 26489717

Clostridium difficile recurrent infection: possible implication of TA systems. Gil F, Pizarro-Guajardo M, Álvarez R, Garavaglia M, Paredes-Sabja D.Future Microbiol. 2015;10(10):1649-57. doi: 10.2217/fmb.15.94. Epub 2015 Oct 6.PMID: 26439907 Review.

Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes. Plaza-Garrido Á, Miranda-Cárdenas C, Castro-Córdova P, Olguín-Araneda V, Cofré-Araneda G, Hernández-Rocha C, Carman R, Ibáñez P, Fawley WN, Wilcox MH, Gil F, Calderón IL, Fuentes JA, Guzmán-Durán AM, Alvarez-Lobos M, Paredes-Sabja D.Anaerobe. 2015 Dec;36:30-8. doi: 10.1016/j.anaerobe.2015.09.005. Epub 2015 Sep 25.PMID: 26403333

Motility modulation by the small non-coding RNA SroC in Salmonella Typhimurium. Fuentes DN, Calderón PF, Acuña LG, Rodas PI, Paredes-Sabja D, Fuentes JA, Gil F, Calderón IL.FEMS Microbiol Lett. 2015 Sep;362(17):fnv135. doi: 10.1093/femsle/fnv135. Epub 2015 Aug 19.PMID: 26293911

Analysis of Vibrio vulnificus Infection Risk When Consuming Depurated Raw Oysters. Deng K, Wu X, Fuentes C, Su YC, Welti-Chanes J, Paredes-Sabja D, Torres JA.J Food Prot. 2015 Jun;78(6):1113-8. doi: 10.4315/0362-028X.JFP-14-421.PMID: 26038900

Location and stoichiometry of the protease CspB and the cortex-lytic enzyme SleC in Clostridium perfringens spores. Banawas S, Korza G, Paredes-Sabja D, Li Y, Hao B, Setlow P, Sarker MR.Food Microbiol. 2015 Sep;50:83-7. doi: 10.1016/j.fm.2015.04.001. Epub 2015 Apr 8.PMID: 25998819

Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi. Valenzuela LM, Hidalgo AA, Rodríguez L, Urrutia IM, Ortega AP, Villagra NA, Paredes-Sabja D, Calderón IL, Gil F, Saavedra CP, Mora GC, Fuentes JA.Infect Genet Evol. 2015 Jul;33:131-42. doi: 10.1016/j.meegid.2015.04.021. Epub 2015 Apr 23.PMID: 25913156

Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores. Díaz-González F, Milano M, Olguin-Araneda V, Pizarro-Cerda J, Castro-Córdova P, Tzeng SC, Maier CS, Sarker MR, Paredes-Sabja D.J Proteomics. 2015 Jun 18;123:1-13. doi: 10.1016/j.jprot.2015.03.035. Epub 2015 Apr 4.PMID: 25849250 Free PMC article.

The inhibitory effects of sorbate and benzoate against Clostridium perfringens type A isolates. Alnoman M, Udompijitkul P, Paredes-Sabja D, Sarker MR.Food Microbiol. 2015 Jun;48:89-98. doi: 10.1016/j.fm.2014.12.007. Epub 2014 Dec 24.PMID: 25790996

[Clostridium difficile spores and its relevance in the persistence and transmission of the infection]. Barra-Carrasco J, Hernández-Rocha C, Ibáñez P, Guzmán-Durán AM, Álvarez-Lobos M, Paredes-Sabja D.Rev Chilena Infectol. 2014 Dec;31(6):694-703. doi: 10.4067/S0716-10182014000600010.PMID: 25679927 Free article. Review. Spanish.

[Performance of prognostic index in severe Clostridium difficile-associated infection: retrospective analysis in a university hospital]. Hernández-Rocha C, Tejos Sufan R, Plaza-Garrido Á, Barra-Carrasco J, Agüero Luengo C, Inostroza Levy G, Ibáñez Lazo P, Guzmán-Durán AM, Paredes-Sabja D, Molina Pezoa ME, Álvarez-Lobos M.Rev Chilena Infectol. 2014 Dec;31(6):659-65. doi: 10.4067/S0716-10182014000600003.PMID: 25679920 Free article. Spanish.

CysB-dependent upregulation of the Salmonella Typhimurium cysJIH operon in response to antimicrobial compounds that induce oxidative stress. Álvarez R, Neumann G, Frávega J, Díaz F, Tejías C, Collao B, Fuentes JA, Paredes-Sabja D, Calderón IL, Gil F.Biochem Biophys Res Commun. 2015 Feb 27;458(1):46-51. doi: 10.1016/j.bbrc.2015.01.058. Epub 2015 Jan 28.PMID: 25637663

Updates on the sporulation process in Clostridium species. Talukdar PK, Olguín-Araneda V, Alnoman M, Paredes-Sabja D, Sarker MR.Res Microbiol. 2015 May;166(4):225-35. doi: 10.1016/j.resmic.2014.12.001. Epub 2014 Dec 23.PMID: 25541348 Review.

Survival of Clostridium difficile spores at low temperatures. Deng K, Plaza-Garrido A, Torres JA, Paredes-Sabja D.Food Microbiol. 2015 Apr;46:218-221. doi: 10.1016/j.fm.2014.07.022. Epub 2014 Aug 23.PMID: 25475288

Recent advances in germination of Clostridium spores. Olguín-Araneda V, Banawas S, Sarker MR, Paredes-Sabja D.Res Microbiol. 2015 May;166(4):236-43. doi: 10.1016/j.resmic.2014.07.017. Epub 2014 Aug 15.PMID: 25132133 Review.

New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates. Udompijitkul P, Alnoman M, Banawas S, Paredes-Sabja D, Sarker MR.Food Microbiol. 2014 Dec;44:24-33. doi: 10.1016/j.fm.2014.04.011. Epub 2014 May 6.PMID: 25084641

Clostridium difficile spore biology: sporulation, germination, and spore structural proteins. Paredes-Sabja D, Shen A, Sorg JA.Trends Microbiol. 2014 Jul;22(7):406-16. doi: 10.1016/j.tim.2014.04.003. Epub 2014 May 7.PMID: 24814671 Free PMC article. Review.

Clostridium difficile spores: a major threat to the hospital environment. Barra-Carrasco J, Paredes-Sabja D.Future Microbiol. 2014;9(4):475-86. doi: 10.2217/fmb.14.2.PMID: 24810347 Review.

Characterization of the collagen-like exosporium protein, BclA1, of Clostridium difficile spores. Pizarro-Guajardo M, Olguín-Araneda V, Barra-Carrasco J, Brito-Silva C, Sarker MR, Paredes-Sabja D.Anaerobe. 2014 Feb;25:18-30. doi: 10.1016/j.anaerobe.2013.11.003. Epub 2013 Nov 21.PMID: 24269655

The Clostridium perfringens germinant receptor protein GerKC is located in the spore inner membrane and is crucial for spore germination. Banawas S, Paredes-Sabja D, Korza G, Li Y, Hao B, Setlow P, Sarker MR.J Bacteriol. 2013 Nov;195(22):5084-91. doi: 10.1128/JB.00901-13. Epub 2013 Sep 6.PMID: 24013629 Free PMC article.

The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly. Barra-Carrasco J, Olguín-Araneda V, Plaza-Garrido A, Miranda-Cárdenas C, Cofré-Araneda G, Pizarro-Guajardo M, Sarker MR, Paredes-Sabja D.J Bacteriol. 2013 Sep;195(17):3863-75. doi: 10.1128/JB.00369-13. Epub 2013 Jun 21.PMID: 23794627 Free PMC article.

High hydrostatic pressure-induced inactivation of bacterial spores. Sarker MR, Akhtar S, Torres JA, Paredes-Sabja D.Crit Rev Microbiol. 2015 Feb;41(1):18-26. doi: 10.3109/1040841X.2013.788475. Epub 2013 Apr 30.PMID: 23631742 Review.

Diagnostic accuracy of a multiplex real-time PCR to predict Clostridium difficile ribotype 027. Barra-Carrasco J, Hernández-Rocha C, Miranda-Cárdenas C, Álvarez-Lobos M, Guzmán Durán AM, Paredes-Sabja D.Anaerobe. 2013 Aug;22:115-7. doi: 10.1016/j.anaerobe.2013.04.002. Epub 2013 Apr 17.PMID: 23603384 No abstract available.

Unique regulatory mechanism of sporulation and enterotoxin production in Clostridium perfringens. Ohtani K, Hirakawa H, Paredes-Sabja D, Tashiro K, Kuhara S, Sarker MR, Shimizu T.J Bacteriol. 2013 Jun;195(12):2931-6. doi: 10.1128/JB.02152-12. Epub 2013 Apr 12.PMID: 23585540 Free PMC article.

Inactivation strategy for Clostridium perfringens spores adhered to food contact surfaces. Udompijitkul P, Alnoman M, Paredes-Sabja D, Sarker MR.Food Microbiol. 2013 Jun;34(2):328-36. doi: 10.1016/j.fm.2013.01.003. Epub 2013 Jan 12.PMID: 23541199

Prospective comparison of a commercial multiplex real-time polymerase chain reaction and an enzyme immunoassay with toxigenic culture in the diagnosis of Clostridium difficile-associated infections. Hernández-Rocha C, Barra-Carrasco J, Álvarez-Lobos M, Paredes-Sabja D, Guzmán-Durán AM.Diagn Microbiol Infect Dis. 2013 Apr;75(4):361-5. doi: 10.1016/j.diagmicrobio.2012.12.010. Epub 2013 Feb 12.PMID: 23415540

Proteases and sonication specifically remove the exosporium layer of spores of Clostridium difficile strain 630. Escobar-Cortés K, Barra-Carrasco J, Paredes-Sabja D.J Microbiol Methods. 2013 Apr;93(1):25-31. doi: 10.1016/j.mimet.2013.01.016. Epub 2013 Feb 4.PMID: 23384826

Atypical presentation of pseudomembranous colitis localized in adenomatous polyps. Hernández-Rocha C, Barra-Carrasco J, Guzmán AM, Paredes-Sabja D, Lezcano G, Zoroquiaín P, Alvarez-Lobos M.World J Gastroenterol. 2013 Jan 14;19(2):316-8. doi: 10.3748/wjg.v19.i2.316.PMID: 23345958 Free PMC article.

[Clostridium difficile associated infections: an updated view]. Hernández-Rocha C, Naour S, Alvarez-Lobos M, Paredes-Sabja D.Rev Chilena Infectol. 2012 Aug;29(4):434-45. doi: 10.4067/S0716-10182012000400011.PMID: 23096544 Free article. Review. Spanish.

Clostridium difficile spore-macrophage interactions: spore survival. Paredes-Sabja D, Cofre-Araneda G, Brito-Silva C, Pizarro-Guajardo M, Sarker MR.PLoS One. 2012;7(8):e43635. doi: 10.1371/journal.pone.0043635. Epub 2012 Aug 27.PMID: 22952726 Free PMC article.

Molecular basis of early stages of Clostridium difficile infection: germination and colonization. Sarker MR, Paredes-Sabja D.Future Microbiol. 2012 Aug;7(8):933-43. doi: 10.2217/fmb.12.64.PMID: 22913353 Review.

Epidemic Clostridium difficile ribotype 027 in Chile. Hernández-Rocha C, Barra-Carrasco J, Pizarro-Guajardo M, Ibáñez P, Bueno SM, Sarker MR, Guzman AM, Alvarez-Lobos M, Paredes-Sabja D.Emerg Infect Dis. 2012 Aug;18(8):1370-2. doi: 10.3201/eid1808.120211.PMID: 22840230 Free PMC article. No abstract available.

Effects of wet heat treatment on the germination of individual spores of Clostridium perfringens. Wang G, Paredes-Sabja D, Sarker MR, Green C, Setlow P, Li YQ.J Appl Microbiol. 2012 Oct;113(4):824-36. doi: 10.1111/j.1365-2672.2012.05387.x. Epub 2012 Aug 2.PMID: 22776375 Free article.

Adherence of Clostridium difficile spores to Caco-2 cells in culture. Paredes-Sabja D, Sarker MR.J Med Microbiol. 2012 Sep;61(Pt 9):1208-1218. doi: 10.1099/jmm.0.043687-0. Epub 2012 May 17.PMID: 22595914

Interactions between Clostridium perfringens spores and Raw 264.7 macrophages. Paredes-Sabja D, Sarker MR.Anaerobe. 2012 Feb;18(1):148-56. doi: 10.1016/j.anaerobe.2011.12.019. Epub 2011 Dec 24.PMID: 22209938

Inhibitory effects of nisin against Clostridium perfringens food poisoning and nonfood-borne isolates. Udompijitkul P, Paredes-Sabja D, Sarker MR.J Food Sci. 2012 Jan;77(1):M51-6. doi: 10.1111/j.1750-3841.2011.02475.x. Epub 2011 Dec 2.PMID: 22132724

Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity. Wang G, Zhang P, Paredes-Sabja D, Green C, Setlow P, Sarker MR, Li YQ.J Appl Microbiol. 2011 Nov;111(5):1212-23. doi: 10.1111/j.1365-2672.2011.05135.x. Epub 2011 Sep 14.PMID: 21883730 Free article.

Clostridium perfringens tpeL is expressed during sporulation. Paredes-Sabja D, Sarker N, Sarker MR.Microb Pathog. 2011 Nov;51(5):384-8. doi: 10.1016/j.micpath.2011.05.006. Epub 2011 Jul 23.PMID: 21810463

Host serum factor triggers germination of Clostridium perfringens spores lacking the cortex hydrolysis machinery. Paredes-Sabja D, Sarker MR.J Med Microbiol. 2011 Dec;60(Pt 12):1734-1741. doi: 10.1099/jmm.0.031575-0. Epub 2011 Jul 28.PMID: 21799201

Germination response of spores of the pathogenic bacterium Clostridium perfringens and Clostridium difficile to cultured human epithelial cells. Paredes-Sabja D, Sarker MR.Anaerobe. 2011 Apr;17(2):78-84. doi: 10.1016/j.anaerobe.2011.02.001. Epub 2011 Feb 12.PMID: 21315167

Germination of spores of Bacillales and Clostridiales species: mechanisms and proteins involved. Paredes-Sabja D, Setlow P, Sarker MR.Trends Microbiol. 2011 Feb;19(2):85-94. doi: 10.1016/j.tim.2010.10.004. Epub 2010 Nov 27.PMID: 21112786 Review.

Effect of the cortex-lytic enzyme SleC from non-food-borne Clostridium perfringens on the germination properties of SleC-lacking spores of a food poisoning isolate. Paredes-Sabja D, Sarker MR.Can J Microbiol. 2010 Nov;56(11):952-8. doi: 10.1139/w10-083.PMID: 21076486

Inorganic phosphate and sodium ions are cogerminants for spores of Clostridium perfringens type A food poisoning-related isolates. Paredes-Sabja D, Udompijitkul P, Sarker MR.Appl Environ Microbiol. 2009 Oct;75(19):6299-305. doi: 10.1128/AEM.00822-09. Epub 2009 Aug 7.PMID: 19666724 Free PMC article.

The protease CspB is essential for initiation of cortex hydrolysis and dipicolinic acid (DPA) release during germination of spores of Clostridium perfringens type A food poisoning isolates. Paredes-Sabja D, Setlow P, Sarker MR.Microbiology (Reading). 2009 Oct;155(Pt 10):3464-3472. doi: 10.1099/mic.0.030965-0. Epub 2009 Jul 23.PMID: 19628563

Further characterization of Clostridium perfringens small acid soluble protein-4 (Ssp4) properties and expression. Li J, Paredes-Sabja D, Sarker MR, McClane BA.PLoS One. 2009 Jul 17;4(7):e6249. doi: 10.1371/journal.pone.0006249.PMID: 19609432 Free PMC article.

Clostridium perfringens sporulation and its relevance to pathogenesis. Paredes-Sabja D, Sarker MR.Future Microbiol. 2009 Jun;4(5):519-25. doi: 10.2217/fmb.09.31.PMID: 19492963

GerO, a putative Na+/H+-K+ antiporter, is essential for normal germination of spores of the pathogenic bacterium Clostridium perfringens. Paredes-Sabja D, Setlow P, Sarker MR.J Bacteriol. 2009 Jun;191(12):3822-31. doi: 10.1128/JB.00158-09. Epub 2009 Apr 10.PMID: 19363115 Free PMC article.

Role of GerKB in germination and outgrowth of Clostridium perfringens spores. Paredes-Sabja D, Setlow P, Sarker MR.Appl Environ Microbiol. 2009 Jun;75(11):3813-7. doi: 10.1128/AEM.00048-09. Epub 2009 Apr 10.PMID: 19363077 Free PMC article.

Germination of spores of Clostridium difficile strains, including isolates from a hospital outbreak of Clostridium difficile-associated disease (CDAD). Paredes-Sabja D, Bond C, Carman RJ, Setlow P, Sarker MR.Microbiology (Reading). 2009 Apr;155(4):1376. doi: 10.1099/mic.0.30214-0.PMID: 33202523 No abstract available.

Strategy to inactivate Clostridium perfringens spores in meat products. Akhtar S, Paredes-Sabja D, Torres JA, Sarker MR.Food Microbiol. 2009 May;26(3):272-7. doi: 10.1016/j.fm.2008.12.011. Epub 2009 Jan 7.PMID: 19269568

SleC is essential for cortex peptidoglycan hydrolysis during germination of spores of the pathogenic bacterium Clostridium perfringens. Paredes-Sabja D, Setlow P, Sarker MR.J Bacteriol. 2009 Apr;191(8):2711-20. doi: 10.1128/JB.01832-08. Epub 2009 Feb 13.PMID: 19218389 Free PMC article.